Eppendorf BioSpectrometer® basic

Eppendorf BioSpectrometer® basic
Eppendorf BioSpectrometer® basic
Eppendorf BioSpectrometer® basic
Eppendorf BioSpectrometer® basic
Eppendorf BioSpectrometer® basic
Eppendorf BioSpectrometer® basic
Eppendorf BioSpectrometer® basic
Eppendorf BioSpectrometer® basic
Eppendorf BioSpectrometer® basic
Eppendorf BioSpectrometer® basic
Eppendorf BioSpectrometer® basic
Eppendorf BioSpectrometer® basic
Eppendorf BioSpectrometer® basic
Eppendorf BioSpectrometer® basic
Eppendorf BioSpectrometer® basic
Eppendorf BioSpectrometer® basic
Eppendorf BioSpectrometer® basic
Eppendorf BioSpectrometer® basic
Eppendorf BioSpectrometer® basic
Eppendorf BioSpectrometer® basic
Eppendorf BioSpectrometer® basic
Eppendorf BioSpectrometer® basic
Eppendorf BioSpectrometer® basic
Eppendorf BioSpectrometer® basic
Eppendorf BioSpectrometer® basic
Eppendorf BioSpectrometer® basic
Eppendorf BioSpectrometer® basic

You need a scan?
The Eppendorf BioSpectrometer® basic makes your work easier: convenient handling, guided software procedures, operation directly on the device, preprogrammed methods, automatic evaluation of measurement data, and clear presentation of results. More information

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Product Information

Building on the legacy of the Eppendorf BioPhotometer D30, the Eppendorf BioSpectrometer basic shares its simplicity, flexibility and reliability. In addition to the Biophotometer D30, the Biospectrometer basic has freely selectable wavelengths from 200 – 830 nm (selectable in 1 nm increments). This allows the user to define the parameters of each assay, whether it be a scan, a single-wavelength measurement or a multi-wavelength measurement. The Eppendorf BioSpectrometer basic caters to more disciplines as the wavelengths are not pre-defined and allows for non-routine assays or new assays to be easily programmed (in addition to the pre-programmed routine methods). The Eppendorf BioSpectrometer basic is a robust yet flexible ˝workhorse˝.

Looking for the target?


The Eppendorf µCuvette is optimized for very low sample volumes. The hydrophobic surface coating on quartz glas enables convenient sample loading.

Eppendorf BioSpectrometer® basic

Left? Right? Up? Down?


Clear indications for positioning the cuvette for convenient handling and safe results.

Eppendorf BioSpectrometer® basic

Believing in raw data?


In general, the purity of a DNA solution is easily defined using purity ratios. It is possible that reduced purity may be attributed to the fact that the sample is solubilized in water instead of in buffer. Since water and buffer have different properties, the absorbance of the sample being measured will be affected by this disparity.
For this reason, the BioSpectrometer is capable of recording a “Purity Scan” which displays the absorbance properties of a sample graphically, thus enabling fast and simple visual quality control.

Eppendorf BioSpectrometer® basic

Looking for easy-to-read results?


In addition, all important raw data pertaining to the DNA sample are displayed in table format. The table optionally lists the purity ratios (A260/A230 and A260/A280), the absorbance values (raw data), as well as the background absorbance, e.g. at 320 nm.
Thus, the two display formats complement each other perfectly to allow for an evaluation of the quality of a DNA sample.

Eppendorf BioSpectrometer® basic

Annoyed by uncomfortable handling?


Ergonomics is far beyond the »ergonomically designed chair«. Eppendorf started to optimize the laboratory devices regarding ergonomics already in the early 1970s.
In 2003, we started the PhysioCare Concept, focusing on ergonomic liquid handling devices like our pipettes.
Nowadays, the Eppendorf PhysioCare Concept is broaden up to include further laboratory products as a holistic solution to harmonize the workflow in your laboratory with your health and well-being, e.g. for the Eppendorf BioSpectrometer:
› Guided software for easy handling
› Clear indications at cuvette shaft for convenient handling
› Direct keys for convenient handling
› Small footprint to enable position in direct arm reach at your bench
› Standardized »user-interface concept« for intuitive handling of devices

Further information about the Eppendorf PhysioCare Concept:
www.eppendorf.com/physiocare/

Eppendorf BioSpectrometer® basic

Struggling with paper-based documentation?


The eLABJournal Electronic Lab Notebook offers an intuitive and flexible solution to document research and track research data like BioSpectrometer–based values. Improve efficiency when documenting, organizing, searching, and archiving collected data. With the free eLABJournal add-ons, you can extend the functionality of eLABJournal to a fully integrated Laboratory Information Management System.

Start a free 30-day trial!
Visit www.eLABJournal.com

Eppendorf BioSpectrometer® basic

Features

  • Absorbance measurement for one or more wavelengths, recording of wavelength scans from 200 nm to 830 nm (increment: 1 nm)
  • Automatic evaluation and storage of measurement data with clear presentation of results
  • Spectral graph to display sample purity with automatic ratio calculation
  • Freely programmable applications with evaluation via factor, standard or standard series and freely programmable parameters
  • Two-wavelength method with substraction or division evaluation
  • Integrated application and results memory
  • Guided software process to minimize errors
  • Integrated self-test and calibration history
  • Data transfer via USB interface, Ethernet or via e-mail and direct printout of results
  • Data output as *.PNG screenshots, Microsoft® Excel®, or secure PDF files to a standard USB flash drive or via email

Applications

  • Nucleic acid quantification
  • Direct protein quantification (UV 280 nm)
  • Microvolume measurement via µCuvette G1.0 for highly concentrated samples
  • Bacteria growth measurement (OD 600)
  • Colorimetric assays for protein quantification, e.g., BCA, Bradford, Lowry
  • Evaluation of FOI for dye-labeled biomolecules (nucleic acid or proteins)
  • Free selectable wavelengths, e.g., 340 nm: assays using NADPH or NAPH, 405 nm: assays using para-nitrophenol, 420 nm: assays using ortho-nitrophenol, 490 nm: colorimetric fructose determination, 490 nm: cytoxicity endpoint assay
  • Transmission measurement
 Eppendorf BioSpectrometer® basic
Absorption measuring range0 A – 3.0 A (260 nm)
BSA concentration range (UV 280 nm) µCuvette® G1.0758 ng/µL – 45,450 ng/µL
BSA concentration range (UV 280 nm) UVette® 10 mm76 ng/µL – 4,545 ng/µL
BSA concentration range (UV 280 nm) UVette® 2 mm379 ng/µL – 22,725 ng/µL
BSA concentration range (for 1 to 10 mm path length, calculation factor for BSA = 1.515 µg/µL)76 ng/µL – 45,450 ng/µL
Cuvette shaft12.5 mm × 12.5 mm
Detector TypeCMOS photodiode array, 1024 pixel
Emission wavelength I fluorescence
Emission wavelength II fluorescence
Excitation wavelength fluorescence
Fluorescence measuring range
Light source absorptionXenon flash lamp
Light source fluorescence
Measuring principle absorptionSingle-beam absorption spectrophotometer with reference beam
Measuring principle fluorescence
Method memory>100 method programs
Operator guidance languageSpanish, Italian, French, English, German, Japanese
Power consumptionApprox. 15 W during operating step
Approx. 5 W with dimmed display
Random error absorption≤0.002 at A = 0
≤0.005 (0.5 %) at A = 1
Random error fluorescence
Random error wavelength≤0.5 nm
Random temperature error
Sample light path length8.5 mm
Scanningyes
Smallest step size1 nm
Spectral bandwidth≤4 nm
Systematic error absorption±1 % (A = 1)
Systematic error wavelength±1 nm
Systematic temperature error
Wavelength Selection Increment1 nm
Wavelength range200 nm – 830 nm
Wavelength range absorptionScan (nm): 200 – 830 at 1 nm increments
dsDNA concentration range (UV 260 nm) µCuvette® G1.025 ng/µL – 1,500 ng/µL
dsDNA concentration range (UV 260 nm) UVette® 10 mm2.5 ng/µL – 150 ng/µL
dsDNA concentration range (UV 260 nm) UVette® 2 mm12.5 ng/µL – 750 ng/µL
dsDNA concentration range (for 1 to 10 mm path length)2.5 ng/µL – 1,500 ng/µL
Interfaces› USB master: for USB stick and thermal printer DPU-S445
› USB slave for connecting to a PC (All functions available without PC)
› Serial interface RS-232: for thermal printer DPU-414
› Ethernet interface RJ45: For connecting to a network printer or emailing data outputs directly off instrument
Power supply100 – 120 V, 50 – 60 Hz
Dimensions (W × D × H)295 × 400 × 150 mm / 11.6 × 15.7 × 6 in
Weight w/o accessories5.4 kg
Interval between two measuring points
Measuring time range
Adjustable temperature range
Eppendorf BioSpectrometer® basic
Video: Eppendorf BioSpectrometer® basic – Video – University of East London

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